LB1001 Effectiveness and differentially expressed genes analysis of melanoma cells treated with cold atmospheric plasma

Cold atmospheric plasma (CAP) has attracted increasing attention due to its anti-bacterial and anti-tumor effects. Melanoma is an aggressive malignancy that increasingly common with a poor prognosis. By evaluating cell viability, detecting cells apoptosis, this study explored the differences of biological effect between melanoma human keratinocyte lines (HaCaT) treated CAP, combining reactive species injection efficiency tumor physiological characteristics system analyzed safety CAP. The results showed under same condition, CAP more significant on in inhibiting proliferation apoptosis. Based study, RNA-sequencing was performed further elucidate differential gene expression cells. besides MAPK p53 apoptosis pathways, autophagy necroptosis also had important roles CAP-induced death. Our provided genomic resources for mechanism cells, particular, new insights into molecular mechanisms necroptosis.